黑水虻作为水产饲料蛋白质原料的研究进展Black Soldier Fly(Hermetia illucens) as Protein Raw Material of Aquatic Feed
吉红,夏濛,胡泽超
摘要(Abstract):
黑水虻(Hermetia illucens)是一种原产于南美洲的腐食性昆虫,它食性广、生长迅速、繁殖能力强,因其幼虫具有蛋白质含量丰富和氨基酸组成均衡等特点,被认为是极具潜力的新型水产饲料蛋白质来源。研究表明,黑水虻作为饲料原料,能够部分甚至完全替代水产饲料中的鱼粉或豆粕,具有良好的应用前景。当前,黑水虻主要以全脂虫粉、脱脂虫粉和预蛹粉等三种形式添加于水产配合饲料中。由于营养特性不同,针对不同的养殖对象应选择相应的黑水虻饲料产品和适宜的添加量,以获得最佳的养殖效果。文章综述了黑水虻的生物学特性、营养价值、影响其生产的因素及其不同产品类型在水产配合饲料中应用的最新研究进展,并就黑水虻产品作为水产饲料蛋白质原料应关注的问题进行了讨论,旨在为其产业建设和高效利用提供参考资料。
关键词(KeyWords): 黑水虻;蛋白源;水产饲料;水产养殖动物;营养价值
基金项目(Foundation): 国家重点研发计划“蓝色粮仓科技创新”重点专项[2019YFD0900200]
作者(Author): 吉红,夏濛,胡泽超
DOI: 10.13302/j.cnki.fi.2023.16.001
参考文献(References):
- [1] FAO. The state of world fisheries and aquaculture 2022[M]. Food and Agriculture Organization of the United Nations, Rome, 2022.
- [2] BRUNI L, GIULIA S, YARA H, et al. Is it possible to cut down fishmeal and soybean meal use in aquafeed limiting the negative effects on rainbow trout(Oncorhynchus mykiss)fillet quality and consumer acceptance?[J]. Aquaculture, 2021, 543:736996.
- [3] FAO. The state of world fisheries and aquaculture 2020[M]. Rome,2020.
- [4] HARDY R W. Utilization of plant proteins in fish diets:effects of global demand and supplies of fishmeal[J]. Aquaculture Research,2010, 41(5):770-776.
- [5] SANTOS J F S, NAVAL L P. Spatial and temporal dynamics of water footprint for soybean production in areas of recent agricultural expansion of the Brazilian savannah(Cerrado)[J]. Journal of Cleaner Production, 2020, 251:119482.
- [6] CHENG W, CHIU C S, GUU Y K, et al. Expression of recombinant phytase of Bacillus subtilis E20 in Escherichia coliHMS 174and improving the growth performance of white shrimp, Litopenaeus vannamei, juveniles by using phytasepretreated soybean meal-containing diet[J]. Aquaculture Nutrition, 2013, 19(2):117-127.
- [7] MERRIFIELD D L, OLSEN R E, MYKLEBUST R, et al. Dietary effect of soybean(Glycine max)products on gut histology and microbiota of fish[J]. Soybean and Nutrition, 2011, 231-250. DOI:10.5772/20101.
- [8] SHIU Y L, WONG S L, GUEI W C, et al. Increase in the plant protein ratio in the diet of white shrimp, Litopenaeus vannamei(Boone), using Bacillus subtilis E20-fermented soybean meal as replacement[J]. Aquaculture Research, 2013, 46(2):382-394.
- [9] MERENGUE M, SCHIAVONE A, GAI F, et al. Effect of rearing substrate on growth performance, waste reduction efficiency and chemical composition of black soldier fly(Hermetia illucens)larvae[J]. Journal of the Science of Food and Agriculture, 2018, 98(15):5776-5784.
- [10] SALOMONE R, SAIJA G, MONDELLO G, et al. Environmental impact of food waste bioconversion by insects:application of life cycle assessment to process using Hermetia illucens[J]. Journal of Cleaner Production, 2017, 140(2):890-905.
- [11] DIENER S, ZURBRUGG C, TOCKNER K. Conversion of organic material by black soldier fly larvae:establishing optimal feeding rates[J]. Waste Management&Research, 2009, 27(6):603-610.
- [12]何振伯.黑水虻生物处理技术的应用[J].中国畜牧兽医文摘,2017, 33(11):226.
- [13] HENRY M, GASCO L, PICCOLO G, et al. Review on the use of insects in the diet of farmed fish:past and future[J]. Animal Feed Science and Technology, 2015, 203:1-22.
- [14] MAKKAR H P, TRAN G, HEUZE V, et al. State-of-the-art on use of insects as animal feed[J]. Animal Feed Science and Technology, 2014, 197:1-33.
- [15] WANG G, PENG K, HU J, et al. Evaluation of defatted black soldier fly(Hermetia illucens L.)larvae meal as an alternative protein ingredient for juvenile Japanese seabass(Lateolabrax japonicus)diets[J]. Aquaculture, 2019, 507:144-154.
- [16]胡俊茹,何飞,莫文艳.采食不同有机废弃物黑水虻幼虫饲料价值分析[J].猪业观察, 2019(Z1):76-80.
- [17] COMMISSION REGULATION(EU). Amending AnnexesⅠandⅣto Regulation(EC)No 999/2001 of the European Parliament and of the Council and Annexes X, XIV and XV to Commission Regulation(EU)No 142/2011 as regards the provisions on processed animal protein(Text with EEA relevance)[R]. 2017/893of 24 May 2017.
- [18] LIU X, CHEN X, WANG H, et al. Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly[J]. PLoS One, 2017, 12(8):e0182601.
- [19]李顺才,吉志新,苏绯等.资源昆虫黑水虻的生长特性与科学利用[J].科学种养, 2020(3):55-57.
- [20]赵竟伽,罗强,江明锋.光照因素对黑水虻幼虫生长发育影响初探[J].畜禽业, 2018, 29(12):7-10.
- [21] TOMBERLIN J K, SHEPPARD D C. Factors influencing mating and oviposition of black soldier flies(Diptera:Stratiomyidae)in a colony[J]. Journal of Entomological Science, 2002, 37(4):345-352.
- [22]萧鸿发,王国霞,彭凯,等.黑水虻生物学特点及其应用研究进展[J].广东畜牧兽医科技, 2020, 45(2):27-33.
- [23]姬越,任德珠,叶明强,等.黑水虻对食料中pH值、辣度及盐度的耐受性[J].应用与环境生物学报, 2018, 24(3):636-639.
- [24]杨燕,严欢,赵智勇,等.以黑水虻为媒介处理两种疫病致死猪的安全性检测[J].养猪, 2016(4):85-86.
- [25] BESKIN K V, CHELSEA D H, JONATHAN A C, et al. Larval digestion of different manure types by the black soldier fly(Diptera:Stratiomyidae)impacts associated volatile emissions[J].Waste Management, 2018, 74:213-220.
- [26] LALANDER C, et al. Fate of pharmaceuticals and pesticides in fly larvae composting[J]. Science of The Total Environment,2016, 565:279-286.
- [27]何钊,赵敏,孙龙等.三种食物喂养的黑水虻幼虫营养测定与比较[J].生物资源, 2018, 40(3):240-245.
- [28] HUANG B, ZHANG S, DONG X, et al. Effects of fishmeal replacement by black soldier fly on growth performance, digestive enzyme activity, intestine morphology, intestinal flora and immune response of pearl gentian grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)[J]. Fish and Shellfish Immunology, 2022, 120:497-506.
- [29]马恒甲,冯晓宇,刘凯,等.黑水虻替代鱼粉对乌鳢生长性能、抗氧化及免疫指标的影响[J].饲料研究, 2021, 44(20):48-52.
- [30] KISHAWY A T Y, HAIAM A M, ASMAA W Z, et al. Partial defatted black solider larvae meal as a promising strategy to replace fish meal protein in diet for Nile tilapia(Oreochromis niloticus):Performance, expression of protein and fat transporters,and cytokines related genes and economic efficiency[J]. Aquaculture, 2022, 555:738195.
- [31] TAKAKUWA F, TANABE R, NOMURA S, et al. Availability of black soldier fly meal as an alternative protein source to fish meal in red sea bream(Pagrus major, Temminck&Schlegel)fingerling diets[J]. Aquaculture Research, 2022, 53(1):36-49.
- [32] LI H D, XUE R R, SUN J, et al. Improving flesh quality of grass carp(Ctenopharyngodon idellus)by completely replacing dietary soybean meal with yellow mealworm(Tenebrio molitor)[J]. Animal Nutrition, 2023, 12:375-387.
- [33] HU Z C, LI H D, LIU S, et al. Assessment of black soldier fly(Hermetia illucens)larvae meal as a potential substitute for soybean meal on growth performance and flesh quality of grass carp Ctenopharyngodon idellus[J]. Animal Nutrition, 2023. https://doi.org/10. 1016/j. aninu. 2023. 06. 006.
- [34]蔡腾,李嘉希,李志强,等.黑水虻幼虫氨基酸及其微量元素分析[J].环境昆虫学报, 2021, 43(3):684-689.
- [35] STEJSKAL V, TRAN H Q, PROKESOVáM, et al. Defatted black soldier fly(Hermetia illucens)in pikeperch(Sander lucioperca)diets:Effects on growth performance, nutrient digestibility, fillet quality, economic and environmental sustainability[J].Animal Nutrition, 2023, 12:7-19.
- [36]王斌,邹仕庚,彭运智等.黑水虻在畜禽饲料中的应用研究进展[J].中国畜牧杂志, 2021, 57(6):8-15.
- [37]王宇,闫卉果,周兴华.黑水虻营养价值及其在水产动物生产中研究进展[J].饲料博览, 2020(8):50-54.
- [38] FISCHER H, ROMANO N, RENUKDAS N, et al. Comparing black soldier fly(Hermetia illucens)larvae versus prepupae in the diets of largemouth bass, Micropterus salmoides:effects on their growth, biochemical composition, histopathology, and gene expression[J]. Aquaculture, 2022, 546:737323.
- [39]于怀龙,黄小燕.黑水虻资源化利用研究[J].饲料工业, 2018,39(6):60-64.
- [40] EL-DAKAR M A B.将有机废弃物饲养的黑水虻幼虫用作水产饲料原料的营养价值评定[D].博士学位论文.杨凌:西北农林科技大学, 2021.
- [41] RAMZY R R, EL-DAKAR M A, WANG D, et al. Conversion efciency of lignin-rich olive pomace to produce nutrient-rich insect biomass by black soldier fly larvae, Hermetia illucens[J]. Waste and Biomass Valorization, 2022, 13:893-903.
- [42] SADYKOVA E O, SHUMAKOVA A A, SHESTAKOVA S I, et al. Nutritional and biological value of Hermetia illucens larvae biomass[J]. Voprosy Pitaniia, 2021, 90(2):73-82.
- [43]徐歆歆,赵俊兰,窦永芳,等.利用裂殖壶藻藻渣提升黑水虻油脂营养价值的研究[J].饲料工业, 2019, 40(10):27-32.
- [44] LILAND N S, IRENE B, PEDRO A, et al. Modulation of nutrient composition of black soldier fly(Hermetia illucens)larvae by feeding seaweed-enriched media[J]. PloS One, 2017, 12(8):e0183188.
- [45]唐晓琴,卢杰.黑水虻人工饲养及其应用[J].农业开发与装备,2015(9):152-155.
- [46]陈倩倩,王金荣,王朋,等.中链脂肪酸的生物学功能及其在动物生产中的应用研究进展[J].中国畜牧杂志, 2022, 58(2):41-47.
- [47] JIN X, ZHOU J, RICHEY G, et al. Undecanoic acid, lauric acid,and N-tridecanoic acid inhibit Escherichia coli Persistence and Biofilm Formation[J]. Journal of Microbiology and Biotechnology,2021, 65(11):2275-2278.
- [48] LI S L, JI H, ZHANG B X, et al. Influence of black soldier fly(Hermetia illucens)larvae oil on growth performance, body composition, tissue fatty acid composition and lipid deposition in juvenile Jian carp(Cyprinus carpio var. Jian)[J]. Aquaculture,2016, 465:43-52.
- [49] BELGHIT I, WAAGBO R, LOCK E J, et al. Insect based diets high in lauric acid reduce liver lipids in freshwater Atlantic salmon[J]. Aquaculture Nutrition, 2019, 25(2):343-357.
- [50]陈延娜,卢荣华,杨国坤,等.黑水虻油替代豆油对草鱼生长性能、抗氧化能力和肠道菌群的影响[J].水产学报, 2019, 43(10):2241-2255.
- [51]贾申宗,林梦君,卢荣华,等.黑水虻油替代豆油对淇河鲫生长性能、血清生化指标和肠道消化酶活力的影响[J].河南师范大学学报:自然科学版, 2022, 50(2):129-135.
- [52]郑丽卿,崔锦良,王月晖,等.黑水虻幼体营养成分分析研究[J].甘肃畜牧兽医, 2019, 49(2):55-56.
- [53] EBENEEZAR S, LINGA PRABU D, TEJPAL C S, et al. Nutritional evaluation, bioconversion performance and phylogenetic assessment of black soldier fly(Hermetia illucens, Linn. 1758)larvae valorized from food waste[J]. Environmental Technology&Innovation, 2021, 23:101783.
- [54] ABDEL-GHANY H M, SALEM M E S. Effects of dietary chitosan supplementation on farmed fish, a review[J]. Reviews in Aquaculture, 2020, 12(1):438-452.
- [55] WANG H, REHMAN K, FENG W, et al. Physicochemical structure of chitin in the developing stages of black soldier fly[J]. International Journal of Biological Macromolecules:Structure,Function and Interactions, 2020, 149:901-907.
- [56] CALIGIANI A, MARSEGLIA A, LENI G, et al. Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin[J]. Food Research International, 2018, 105:812-820.
- [57] SPRANGHERS T, OTTOBONI M, KLOOTWIJK C, et al. Nutritional composition of black soldier fly(Hermetia illucens)prepupae reared on different organic waste substrates[J]. Journal of the Science of Food and Agriculture, 2017, 97(5):2594-2600.
- [58] KROECKEL S, HARJES A G E, ROTH I, et al. When a turbot catches a fly:evaluation of a pre-pupae meal of the Black Soldier Fly(Hermetia illucens)as fish meal substitute-Growth performance and chitin degradation in juvenile turbot(Psetta maxima)[J]. Aquaculture, 2012, 364-365(Complete):345-352.
- [59] FISHER H J, COLLINS S A, HANSON C, et al. Black solider fly larvae meal as a protein source in low fish meal diets for Atlantic salmon(Salmo salar)[J]. Aquaculture, 2020, 521:734978.
- [60] LINDSAY G J H, WALTON M J, ADRON J W, et al. The growth of rainbow trout(Salmo gairdneri)given diets containing chitin and its relationship to chitinolytic enzymes and chitin digestibility[J]. Aquaculture, 1984, 37(4):315-334.
- [61] KARLSEN?, AMLUND H, BERG A, et al. The effect of dietary chitin on growth and nutrient digestibility in farmed Atlantic cod, Atlantic salmon and Atlantic halibut[J]. Aquaculture Research, 2017, 48(1):123-133.
- [62] JANSSEN R H, VINCKEN J P, VAN DEN BROEK L A M, et al.Nitrogen-to-protein conversion factors for three edible insects:Tenebrio molitor, alphitobius diaperinus, and Hermetia illucens[J].Journal of Agricultural and Food Chemistry, 2017, 65(11):2275-2278.
- [63] TRAN H Q, NGUYEN T T, PROKESOVA M D, et al. Insight into bioavailability of various insect meals for European perch(Perca fluviatilis):a nutritional and stable isotopic evaluation[J].Aquaculture, 2023, 563:738912.
- [64] BASTO A, MATOS E, VALENTE L M P. Nutritional value of different insect larvae meals as protein sources for European sea bass(Dicentrarchus labrax)juveniles[J]. Aquaculture, 2020, 521:735085.
- [65]王龙,廖业,孙虹霞,等. Cu2+胁迫对黑水虻幼虫抗菌肽组分及抑菌活性的影响[J].环境昆虫学报, 2019, 41(6):1348-1354.
- [66] ANDREU D, RIVAS L. Animal antimicrobial peptides:an overview[J]. Biopolymers, 1998, 47(6):415-433.
- [67] LAI Y P, GALLO R L. AMPed up immunity:how antimicrobial peptides have mμltiple roles in immune defense[J]. Trends in Immunology, 2009, 30(3):131-141.
- [68] MORETTA A, SALVIA R, SCIEUZO C, et al. A bioinformatic study of antimicrobial peptides identified in the black soldier fly(BSF)Hermetia illucens(Diptera:Stratiomyidae)[J]. Science Report, 2020, 10(1):16875.
- [69]张飞骏,韩新才,厉莉,等.黑水虻抗菌肽对曲霉和沙门氏菌的抑制作用及营养成分研究[J].中国饲料, 2023(9):26-29, 37.
- [70]冯群,高嘉敏,夏嫱.黑水虻幼虫抗菌肽粗提物敏感肿瘤细胞株筛选及溶血作用研究[J].医学综述, 2020, 26(6):1214-1218,1223.
- [71]赵启凤.黑水虻抗菌肽诱导及粗提物活性研究[D].硕士学位论文.遵义:遵义医学院, 2012.
- [72]章启慧,李昆太,王会,等.黑水虻抗菌肽研究进展[J].江西农业大学学报, 2022, 44(4):996-1004.
- [73]肖旭全,吴萍,杜瑞雪,等.黑水虻在水产饲料中的研究进展[J].中国饲料, 2023(9):114-119.
- [74] NASER E D S, ROZEN K, ELISSEN H, et al. Bioconversion of different waste streams of animal and vegetal origin and manure by black soldier fly larvae Hermetia illucens.(Diptera:Stratiomyidae)[J]. Insects, 2023, 14(2):204.
- [75] HOSSEINDOUST A, HA S H, MUN J Y, et al. Quality characteristics of black soldier flies produced by different substrates[J]. Insects, 2023, 14:500.
- [76]王慧姣,李峰,张文蕾,等.利用黑水虻幼虫处理中草药渣和花生饼及幼虫营养价值分析[J].河南水产, 2021(1):22-26.
- [77]李峰,张文蕾,郝小雨,等.利用黑水虻处理餐厨垃圾和豆腐渣及幼虫营养价值分析[J].河南水产, 2020(1):21-25.
- [78] TSCHIRNER M, SIMON A. Influence of different growing substrates and processing on the nutrient composition of black soldier fly larvae destined for animal feed[J]. Journal of Insects as Food and Feed, 2015, 1(3):1-12.
- [79] OONINCX D G A B, BROEKHOVEN S, HUIS A, et al. Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products[J]. PLoS One, 2015, 10(12):e0144601.
- [80] KAROL B BARRAGAN-FONSECA, GORT G, DICKE M, et al.Effects of dietary protein and carbohydrate on life-history traits and body protein and fat contents of the black soldier fly Hermetia illucens[J]. Physiological Entomology, 2019, 44(2):148-159.
- [81] NEWTON G L, BOORAM C V, BARKER RW, et al. Dried Hermetia illucens larvae meal as a supplement for swine[J]. Journal of Animal Science, 1977, 44(3):395-400.
- [82] NEWTON G L, SHEPPARD D C, WATSON D W, et al. The black soldier fly, Hermetia illucens, as a manure management/resource recovery tool[C]//Proceedings of the Symposium on the State of the Science of Animal Manure and Waste Management.San Antonio, Texas, USA, 2005.
- [83]魏红芳,曹佩佩,郭建来,等.黑水虻幼虫不同加工方式营养价值评价[J].饲料工业, 2023, 44(8):17-23.
- [84] HUANG C, FENG W, XIONG J, et al. Impact of drying method on the nutritional value of the edible insect protein from black soldier fly(Hermetia illucens L.)larvae:amino acid composition,nutritional value evaluation, in vitro digestibility, and thermal properties[J]. European Food Research and Technology, 2019,245:11-21.
- [85] MONISHA C, LOGANATHAN M. Impact of drying methods on the physicochemical properties and nutritional composition of defatted black soldier fly(Hermetia illucens)pre-pupae flour[J]. Journal of Food Processing and Preservation, 2022, 46(1):e16184.
- [86] SCHIAVONE A, MARCO M D, MARTíNEZ S, et al. Nutritional value of a partially defatted and a highly defatted black soldier fly larvae(Hermetia illucens L.)meal for broiler chickens:apparent nutrient digestibility, apparent metabolizable energy and apparent ileal amino acid digestibility[J]. Journal of Animal Science and Biotechnology, 2017, 8(1):51.
- [87] LAURENT S, JURY V, LAMBALLERIE M, et al. Effect of two defatting processes on the physicochemical and flow properties of Hermetia illucens and Tenebrio molitor larvae powders[J]. Journal of Food Processing and Preservation, 2022, 46(10):e16853.
- [88]黄文庆,黄燕华,米海峰,等. 3种动物蛋白质源替代鱼粉对草鱼生长性能、肌肉营养成分、消化酶活性、血清生化和抗氧化指标的影响[J].动物营养学报, 2019, 31(5):2187-2200.
- [89] SANTOS D K M D, ODAIR R D F, CESAR A O, et al. Full-fat black soldier fly larvae meal in diet for tambaqui, Colossoma macropomum:digestibility, growth performance and economic analysis of feeds[J]. Animals, 2023, 13(3):360.
- [90] KHIEOKHAJONKHET A, PHUSANISA U, KHANITTA R, et al.Replacement of fish meal by black soldier fly larvae meal in diet for goldfish Carassius auratus:growth performance, hematology,histology, total carotenoids, and coloration[J]. Aquaculture, 2022,561:738618.
- [91] URBAN J,?TYS D, SERGEJEVOVáM, et al. Expertomica fishgui:comparison of fish skin colour[J]. Journal of Applied Ichthyology, 2012, 29(1):172-180.
- [92] AGBOHESSOU P S, MANDIKI S N M, GOUGB'EDJI A, et al.Efficiency of fatty acid-enriched dipteran-based meal on husbandry, digestive activity and immunological responses of Nile tilapia Oreochromis niloticus juveniles[J]. Aquaculture, 2021, 545:737193.
- [93] EGGINK K M, PER BOVBJERG PEDERSEN, IVAR LUND, et al. Chitin digestibility and intestinal exochitinase activity in Nile tilapia and rainbow trout fed different black soldier fly larvae meal size fractions[J]. Aquaculture Research, 2022, 53(16):5536-5546.
- [94]孟林.黑水虻幼虫粉替代鱼粉对加州鲈生长性能的影响[J].江西水产科技, 2023(1):22-23.
- [95] JIANG B, YANXIN SUN, WEI LI, et al. Effects of dietary black soldier fly(Hermetia illucens Linnaeus)on the disease resistance of juvenile grouper(Epinephelus coioides)[J]. Fish&Shellfish Immunology, 2022, 123:136-141.
- [96] ABDEL-LATIF H M R, MOHSEN ABDEL-TAWWAB, RIAD H K, et al. Black soldier fly(Hermetia illucens)larvae meal in diets of european seabass:effects on antioxidative capacity, nonspecific immunity, transcriptomic responses, and resistance to the challenge with vibrio alginolyticus[J]. Fish&Shellfish Immunology, 2021, 111:111-118.
- [97] BOLTON C M, MULLER N, HYLAND J, et al. Black soldier fly larval meal with exogenous protease in diets for rainbow trout(Oncorhynchus mykiss)production meeting consumer quality[J].Journal of Agriculture and Food Research, 2021, 6:100232.
- [98]贲玲芝,史雪莹,郭金龙,等.全脂黑水虻幼虫粉替代鱼粉对大菱鲆养殖性能、生理代谢及体色的影响[J].渔业科学进展,2022, 43(2):80-88.
- [99] BRUNI L, VESNA MILANOVI'C, FRANCESCA TULLI, et al.Effect of diets containing full-fat Hermetia illucens on rainbow trout microbiota:a dual cultivation-independent approach with DGGE and NGS[J]. Aquaculture, 2022, 553:738109.
- [100] RIMOLDI S, CHIARA C, FABIO B, et al. Potential of shrimp waste meal and insect exuviae as sustainable sources of chitin for fish feeds[J]. Aquaculture, 2023, 567:739256.
- [101] WETHTHASINGHE P, HANSEN J, N?KLAND D, et al. Fullfat black soldier fly larvae(Hermetia illucens)meal and paste in extruded diets for Atlantic salmon(Salmo salar):Effect on physical pellet quality, nutrient digestibility, nutrient utilization and growth performances[J]. Aquaculture, 2021, 530:735785.
- [102]陈永康,郑朝中,章双,等.黑水虻幼虫粉对凡纳滨对虾生长、免疫和脂质代谢的影响[J].水生生物学报, 2023, 47(2):269-278.
- [103] WANG T, WANG X, SHEHATA A I, Growth performance, physiological and antioxidant capacity responses to dietary fish meal replacement with insect meals for aquaculture:a case study in red claw crayfish(Cherax quadricarinatus)[J]. Aquaculture Research, 2022, 53(10):3853-3864.
- [104]窦碧静,潘燕平, JULIETH M,等.凡纳滨对虾几丁质酶的活性分布及其短波紫外线照射激活作用[J].食品与发酵工业,2023, 49(5):200-206.
- [105]张文锐,刘洪霞,王尊,等.脱脂黑水虻虫粉对青蟹营养品质的影响[J].饲料研究, 2023, 46(5):51-55.
- [106]余维鹏,陈延娜,张玉茹,等. 4种昆虫蛋白粉对草鱼生长和免疫功能的影响[J].饲料工业, 2023, 44(2):81-88.
- [107] LU R H, CHEN Y N, YU W P, et al. Defatted black soldier fly(Hermetia illucens)larvae meal can replace soybean meal in juvenile grass carp(Ctenopharyngodon idellus)diets[J]. Aquaculture Reports, 2020, 18:100520.
- [108]胡玉峰.黑水虻虫粉对鲫鱼抗氧化功能的影响[J].江西水产科技, 2022(4):19-20, 23.
- [109] GEBREMICHAEL A, BALáZS KUCSKA, LáSZLóARDó, et al.Physiological response of grower african catfish to dietary black soldier fly and mealworm meal[J]. Animals, 2023, 13:968.
- [110] FAWOLE F J, ADEOYE A A, TIAMIYU L O, et al. Substituting fishmeal with Hermetia illucens in the diets of African catfish(Clarias gariepinus):effects on growth, nutrient utilization,haemato-physiological response, and oxidative stress biomarker[J]. Aquaculture, 2020, 518:734849.
- [111]邬伊田,王迦源,孔琳惠,等.脱脂黑水虻虫粉对大口黑鲈生长性能、氮磷利用及肝脏抗氧化力的影响[J].饲料工业,2022, 43(22):55-59.
- [112]韩星星,叶坤,王志勇,等.脱脂黑水虻虫粉替代鱼粉对大黄鱼幼鱼生长、体成分、血清生化指标及抗氧化能力的影响[J].中国水产科学, 2020, 27(5):524-535.
- [113] RENNA M, SCHIAVONE A, GAI F, et al. Evaluation of the suitability of a partially defatted black soldier fly(Hermetia illucens L.)larvae meal as ingredient for rainbow trout(Oncorhynchus mykiss Walbaum)diets[J]. Journal of Animal Science Biotechnology, 2017, 8:57.
- [114] TEROVA G, CECCOTTI C, ASCIONE C, et al. Effects of partially defatted Hermetia illucens meal in rainbow trout diet on hepatic methionine metabolism[J]. Animals:an Open Access Journal from MDPI, 2020, 10(6):1059.
- [115]陈燕,张鸣,魏腾飞,等.不同添加水平黑水虻幼虫粉对大菱鲆生长性能和血清生化指标的影响[J].饲料研究, 2023, 46(2):79-83.
- [116] WANG G, PENG K, HU J, et al. Ev