杨露,袁勇军

• 1:浙江万里学院生物与环境学院

摘要(Abstract)：

从大弹涂鱼肠道中分离适用于发酵大弹涂鱼饲料的优良乳酸菌，并优化其发酵条件。采用钙溶圈法分离纯化得到22株乳酸菌，通过对其产酸能力、抑菌能力、耐胆盐能力、耐酸能力、发酵饲料性能进行综合评价，得到一株发酵性能良好的乳酸菌菌株Bp1。结合生化结果和16S rRNA进化分析,菌株Bp1鉴定发酵黏液乳杆菌。以市售普通大弹涂鱼饲料为原料，加入Bp1菌株并对接种量、发酵温度、初始含水量、发酵时间四个因素进行正交试验，确定最佳发酵工艺为接种量2.0%、发酵温度37℃、初始含水量60%、发酵时间3 d，最优条件下发酵饲料的粗蛋白含量为38.15%。研究结果为大弹涂鱼发酵饲料的制备提供了理论基础。

关键词(KeyWords)： 乳酸菌;发酵饲料;分离鉴定;抑菌;大弹涂鱼

Abstract:

Keywords:

基金项目(Foundation): 浙江省基础公益研究计划项目[LGN22D060003];; 浙江省“生物工程”一流学科学生创新项目[CX2022028]

作者(Author): 杨露,袁勇军

DOI: 10.13302/j.cnki.fi.2024.08.013

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