甲壳素在中华鳖养殖中的应用
魏文志,付立霞,黄薛俊,丁建梅
摘要(Abstract)：
在基础饲料中分别添加0(对照组)、0.3%、0.6%、1.2%的甲壳素,用其饲养中华鳖31d后,测定中华鳖增重率、成活率及血清中溶菌酶、酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶的活性。结果表明,添加0.6%的甲壳素能显著促进中华鳖的生长和非特异性的免疫能力。
关键词(KeyWords)： 甲壳素;中华鳖;溶菌酶;酸性磷酸酶;碱性磷酸酶;超氧化物歧化酶
基金项目(Foundation):
作者(Author): 魏文志,付立霞,黄薛俊,丁建梅
参考文献(References)：
- 1胡品虎.稀土甲壳素在河蟹养殖中的应用[J].水产养殖,1999(5):13￣14
- 2向枭,向勇,冯君,等.甲壳素和左旋咪唑对罗非鱼的实验[J].饲料研究,2002(12):4￣6
- 3Kono M,Matsui T,Shimizu C.Effect of chitin,chitosan,and cellulose as diet supplements on the growth of cultured fish[J].Nippon Suisan Gakkaishi,1987,53(1):125￣129
- 4Hultmark D.Insect immunity:Purification and properties of three in-ducible bactericidal proteins from hemolymph of immunized pupae of Hyalophera cecropia[J].European Journal of Biochemistry,1980(106):7￣16
- 5宋善俊.临床医师手册[M].上海:上海科学技术出版社,1991.185￣187;199￣200
- 6邓碧玉,袁勤生,李文杰.改良的连苯三酚自氧化法测定超氧化物歧化酶活性的方法[J].生物化学与生物物理进展,1991,18(2):163
- 7徐春来,汪以真.甲壳素在水产养殖中的应用[J].中国饲料,2005(7):30￣32
- 8Gabriel J H Lindsay,M.J.Walton.The growth of rainbow trout(salmo gairdneri)given diets containing chintin and its relationship to chiti-nolytic enzymes and chintin digestibility[J].Aquaculture,1984(37):315￣334
- 9Shi-Yen Shiau,Yi-Ping Yu.Dietary supplementation of chitin and chitosan depresses growth in tilapia,Oreochromis niloticus×O.aureus[J].Aquaculture,1999(179):439￣446
- 10黄俊明,吴丽明,陈瑞仪.甲壳素和壳聚糖对小鼠免疫调节的研究.广东卫生防疫,1999,25(1):4￣6
- 11张莉,尹燕博,崔尚金,等.甲壳素、油乳剂和蜂胶对鸡新城疫疫苗免疫调节作用的研究[J].中国预防兽医学报,2004,26(2):136￣138
- 12Sakai D K.Repertoire of complement in immunological defense mechanisms of fish[J].Annual Review of Fish Diseases,1992(2):223￣247
- 13M.A.Esteban,A.Cuesta,J.Ortu$o,et al.Immunomodulatory effects of dietary intake of chitin on gilthead seabream(Sparus aurata L.)innate immune system[J].Fish&Shellfish Immunology,2001(11):303￣315